PREVENTING VIRAL INFECTIONS THROUGH THE ACT OF WUDU AND IDENTIFYING ENTEROVIRUSES
Keywords:
Enterovirus (EV), Viral capsid protein 1 (VP1), Molecular TypingAbstract
One of the smallest creatures created by the lord almighty known as the human enteroviruses cause human disease. These intelligent viruses require a faster method of identification for the purposes of diagnosis. The traditional serum neutralization assay for identification produces nonspecific results due to cross-reactivity of the antibodies used. This study evaluates an alternative method for identification through molecular typing of the VP1 capsid region. This process uses reverse transcription PCR (polymerase chain reaction) on extracted unknown viral RNA, followed by nucleotide sequencing of the cDNA of the VP1 capsid region. In this study, molecular typing of the VP1 capsid region was used to identify unknown human polio virus. Human polio virus type 1 was identified, proving that this method of identification was instantaneous.